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Sunday, 14 February, 2010
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Celiac Test Kit
Most frequently asked Questions and Answers re- Biocard Celiac test kit:
Q-What does the test do?
A-The test identifies whether IgA antibodies to transglutaminase – a key marker for celiac disease – are present in the blood sample.
Gluten intolerance (Celiac disease) is a long-term illness
Wheat allergy, which a short-term reaction that simply means the patient, should avoid heavy exposure to wheat.
Q- I have been omitting wheat and gluten food from my diet for several weeks, will your test still show if I have celiac disease?
A - To ensure the best performance you should be eating normally, if you have already begun to omit foods containing gluten the test could produce a negative result. To ensure the best outcome you should be eating foods containing gluten for up to 4-8 weeks before carrying out the test.
Q- How accurate is the test?
A- The biocard test strip uses the same technology as that employed by pathology labs and is as accurate as a lab result.
Q- How do I do the test?
A- Step 1: A finger prick sample of blood is collected in a capillary tube and placed in the vial of diluent solution. Having replaced the cap the vial is rotated in order to mix the blood with the solution.
The test kit includes 2 safety lancets (finger prick devices designed for single operation).
One or two small/medium sized drops of blood dripped in to the small capillary tube containing the diluent are enough to complete the test comfortably
Step 2: Place the test strip in the vial containing the blood sample for five minutes. After this time the test stick should be removed from the vial in order to read the results, as per the instructions shown on the pack insert included in the carton.
Please note that the test kit is supplied with a sterile lancet, alcohol wipe, diluent vials and one test strip and instruction leaflet
Q – Can the test be used more than once, if the result is negative?
A- The test strip can only be used once even if the test is negative
Q – How do I read the results?
A- The test result is positive if a red control line appears in the control field and a second light to dark red line forms in the test field. The test result is negative if a red control line appears in the control field and no red line forms in the test field.
Q- The test is positive, what do I do now?
A- You should consult with your own GP who will carry out further investigations aimed at confirming that the patient has celiac disease.
Q- The test was negative but I still have symptoms. What does this mean?
A- If the test proves negative and symptoms persist or you have any other concerns you should consult your GP as soon as possible.
Q- What is the science behind the test?
A- Previously tests to measure IgA antibodies to tissue transglutaminase (tTG) used either synthetic tTG produced in a laboratory or animal tTG. This test is unique in that it uses the patient’s own tTG.
tTG is present throughout the human body. This particular test extracts tTG from the patient’s red blood cells.
When the solution in the vial is mixed with the patient’s blood sample it breaks down the red blood cells releasing the tTG contained within. If the patient has celiac disease their blood sample will contain IgA antibodies to tTG and these antibodies will recognise the tTG released from the red blood cells and bind themselves to it.
These newly formed IgA-tTG complexes then bind to the test field of the test stick to give a positive result.
Q- I have been avoiding Wheat and other food groups containing gluten, will this affect the test?
A- If you have dropped wheat/gluten containing foods the test may well produce a false negative. If you have been avoiding these foods for over 1 month the test may well produce a negative result. You must re-introduce wheat and gluten containing foods for a period of 6-8 weeks before carrying out the test.
Q- I have been diagnosed with Celiac, can I use this test to monitor the disease?
A- The test is ideal for screening known celiac sufferers and should produce a negative result if the client has been following the correct diet.
Q- I would like to test my baby, as I am Celiac, can I use your test?
A- Ideally the test should not be used on very small children; we would suggest that children should be a minimum of five (5) years of age before using the Biocard test.
Q-What does the test do?
A-The test identifies whether IgA antibodies to transglutaminase – a key marker for celiac disease – are present in the blood sample.
Gluten intolerance (Celiac disease) is a long-term illness
Wheat allergy, which a short-term reaction that simply means the patient, should avoid heavy exposure to wheat.
Q- I have been omitting wheat and gluten food from my diet for several weeks, will your test still show if I have celiac disease?
A - To ensure the best performance you should be eating normally, if you have already begun to omit foods containing gluten the test could produce a negative result. To ensure the best outcome you should be eating foods containing gluten for up to 4-8 weeks before carrying out the test.
Q- How accurate is the test?
A- The biocard test strip uses the same technology as that employed by pathology labs and is as accurate as a lab result.
Q- How do I do the test?
A- Step 1: A finger prick sample of blood is collected in a capillary tube and placed in the vial of diluent solution. Having replaced the cap the vial is rotated in order to mix the blood with the solution.
The test kit includes 2 safety lancets (finger prick devices designed for single operation).
One or two small/medium sized drops of blood dripped in to the small capillary tube containing the diluent are enough to complete the test comfortably
Step 2: Place the test strip in the vial containing the blood sample for five minutes. After this time the test stick should be removed from the vial in order to read the results, as per the instructions shown on the pack insert included in the carton.
Please note that the test kit is supplied with a sterile lancet, alcohol wipe, diluent vials and one test strip and instruction leaflet
Q – Can the test be used more than once, if the result is negative?
A- The test strip can only be used once even if the test is negative
Q – How do I read the results?
A- The test result is positive if a red control line appears in the control field and a second light to dark red line forms in the test field. The test result is negative if a red control line appears in the control field and no red line forms in the test field.
Q- The test is positive, what do I do now?
A- You should consult with your own GP who will carry out further investigations aimed at confirming that the patient has celiac disease.
Q- The test was negative but I still have symptoms. What does this mean?
A- If the test proves negative and symptoms persist or you have any other concerns you should consult your GP as soon as possible.
Q- What is the science behind the test?
A- Previously tests to measure IgA antibodies to tissue transglutaminase (tTG) used either synthetic tTG produced in a laboratory or animal tTG. This test is unique in that it uses the patient’s own tTG.
tTG is present throughout the human body. This particular test extracts tTG from the patient’s red blood cells.
When the solution in the vial is mixed with the patient’s blood sample it breaks down the red blood cells releasing the tTG contained within. If the patient has celiac disease their blood sample will contain IgA antibodies to tTG and these antibodies will recognise the tTG released from the red blood cells and bind themselves to it.
These newly formed IgA-tTG complexes then bind to the test field of the test stick to give a positive result.
Q- I have been avoiding Wheat and other food groups containing gluten, will this affect the test?
A- If you have dropped wheat/gluten containing foods the test may well produce a false negative. If you have been avoiding these foods for over 1 month the test may well produce a negative result. You must re-introduce wheat and gluten containing foods for a period of 6-8 weeks before carrying out the test.
Q- I have been diagnosed with Celiac, can I use this test to monitor the disease?
A- The test is ideal for screening known celiac sufferers and should produce a negative result if the client has been following the correct diet.
Q- I would like to test my baby, as I am Celiac, can I use your test?
A- Ideally the test should not be used on very small children; we would suggest that children should be a minimum of five (5) years of age before using the Biocard test.
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